Overview
Semaglutide is a long-acting GLP-1 receptor agonist modified with a C18 fatty diacid chain attached via a hydrophilic linker to lysine at position 34, enabling albumin binding and dramatically extending its half-life compared to native GLP-1 (approximately 7 days vs. ~2 minutes). It is a structural analog of native human GLP-1 with 94% sequence homology and two modifications that confer resistance to dipeptidyl peptidase-4 (DPP-4) degradation.
Semaglutide has become one of the most widely studied peptides in the context of metabolic research due to its pronounced effects on body weight and glycemic control observed in large-scale clinical programs. Its mechanism involves both peripheral (pancreatic, gastrointestinal) and central nervous system actions, particularly in hypothalamic and brainstem regions governing appetite and energy homeostasis.
Mechanism of Action
Key mechanisms under investigation:
- Activation of GLP-1 receptors on pancreatic β-cells, stimulating glucose-dependent insulin secretion
- Suppression of glucagon release from α-cells in hyperglycemic conditions
- Delayed gastric emptying reducing postprandial glucose excursions
- Central GLP-1R agonism in hypothalamus and area postrema reducing appetite and food intake
- Potential effects on hepatic glucose production and lipid metabolism
- Cardiovascular effects via GLP-1R expression in cardiac and vascular tissues
Specifications
| Full Name | Semaglutide |
| Modification | C18 fatty diacid via Glu-miniPEG linker at Lys³⁴ |
| Molecular Formula | C₁₈₇H₂₉₁N₄₅O₅₉ |
| Molecular Weight | ~4,113.6 Da |
| CAS Number | 910463-68-2 |
| Purity | ≥98% (HPLC verified) |
| Form | Lyophilized powder |
| Appearance | White to off-white powder |
| Storage (lyophilized) | −20°C protected from light |
| Storage (reconstituted) | 2–8°C, use within 4 weeks |
| Solubility | Sterile or bacteriostatic water (pH-adjusted) |
Reconstitution
Reconstitution Guide
Reconstitute with sterile water or bacteriostatic water. Add solvent slowly along the inner vial wall and allow to dissolve by gentle swirling — do not vortex. Semaglutide may require gentle warming to room temperature before reconstitution due to the fatty acid modification. Store reconstituted solution at 2–8°C and use within 4 weeks. Do not freeze reconstituted solution.
Quality & Purity
Every batch of Semaglutide supplied by Trusty Peptide undergoes third-party HPLC testing to verify purity of ≥98%. A Certificate of Analysis (COA) detailing the batch number, test results, and molecular confirmation is included with every order and available on request prior to purchase.
Research Use Only. Semaglutide is sold strictly for in vitro and laboratory research purposes. This product is not intended for human or veterinary use, and is not a drug, food, or cosmetic. It has not been approved by the FDA or any other regulatory body for human consumption or therapeutic use. All handling should be performed by qualified researchers following applicable safety protocols.