Overview
NAD+ (nicotinamide adenine dinucleotide) is an essential coenzyme found in all living cells that plays a central role in redox reactions, energy metabolism, and cellular signaling. It exists in two interconvertible forms — the oxidized NAD+ and reduced NADH — cycling between these states during metabolic processes including glycolysis, the citric acid cycle, and oxidative phosphorylation. Beyond energy metabolism, NAD+ serves as a substrate for sirtuins (SIRT1–7), poly(ADP-ribose) polymerases (PARPs) involved in DNA repair, and CD38, a cyclic ADP-ribose hydrolase involved in calcium signaling.
NAD+ levels decline significantly with age in multiple tissues, and this decline has been linked to metabolic dysfunction, reduced mitochondrial efficiency, and impaired DNA repair capacity in preclinical models. Research into NAD+ repletion via precursors (NMN, NR) and direct NAD+ administration has expanded dramatically, positioning NAD+ as one of the most studied molecules in longevity and aging biology.
Mechanism of Research Interest
- Sirtuin (SIRT1–7) activation — sirtuins are NAD+-dependent deacetylases regulating gene expression, mitochondrial biogenesis (SIRT1/PGC-1α), and stress responses
- PARP1/2 activation consuming NAD+ for single-strand DNA break repair — a major NAD+ drain under oxidative stress
- CD38 regulation — CD38 is a major NAD+-consuming enzyme whose activity increases with age and inflammation, competing with sirtuins
- NAMPT-mediated salvage pathway as the rate-limiting step in NAD+ biosynthesis
- Downstream effects on mitochondrial function, NF-κB inflammatory signaling, and circadian clock regulation via SIRT1
Specifications
| Full Name | Nicotinamide Adenine Dinucleotide (oxidized form) |
| Formula | C₂₁H₂₇N₇O₁₄P₂ |
| MW | 663.4 Da |
| CAS | 53-84-9 |
| Purity | ≥98% HPLC |
| Form | Lyophilized powder |
| Appearance | White to light yellow powder |
| Storage Lyophilized | −20°C in desiccated environment |
| Storage Reconstituted | 2–8°C, use within 2 weeks |
| Solubility | Water (freely soluble) |
Reconstitution
Reconstitution Protocol
Reconstitute with sterile water. NAD+ is highly water-soluble and dissolves rapidly with gentle swirling. Due to the absence of a bacteriostatic preservative, reconstituted NAD+ should be used within 2 weeks and stored at 2–8°C. Do not freeze reconstituted solutions. NAD+ is sensitive to light and oxidation — store lyophilized powder at −20°C in a desiccated, light-protected environment.
Quality & Purity
Every batch of NAD+ undergoes third-party HPLC testing to verify ≥98% purity. COA included with every order.
Research Use Only. NAD+ supplied by Trusty Peptide is intended strictly for laboratory and in vitro research purposes. It is not approved for human or veterinary use, and is not a medicine, drug, or nutritional supplement. By purchasing you confirm you are a qualified researcher using this compound for legitimate scientific research only.